Millersville is an unincorporated community in western Cape Girardeau County, Missouri, United States. It is located six miles (10 km) west of Jackson on Route 72.
Millersville is part of the Cape Girardeau–Jackson, MO-IL Metropolitan Statistical Area.
A post office called Millersville has been in operation since 1866. The community was named after the local Miller family.
The Miller-Seabaugh House and Dr. Seabaugh Office Building was listed on the National Register of Historic Places in 1996.