Guysville is an unincorporated community in southern Rome Township, Athens County, Ohio, United States. Although it is unincorporated, it has a post office, with the ZIP code of 45735.
The town is located on the Hocking River at the junction of U.S. Route 50 and State Route 329.
Guysville was laid out in 1836. A post office has been in operation at Guysville since 1838. The community was named for Guy Barrows, first postmaster.